Elixir Products Investigations » Research report concerning a preliminary study on the anti-herpes activity of herbal extracts in the series of Elixirs
St. Kliment Ohridski University in Sofia, Bulgaria Faculty of Biology
Research report concerning a preliminary study on the anti-herpes activity of herbal extracts in the series of Elixirs
Laboratory of Virology, Military Medical Academy, Sofia, Bulgaria
Introduction
Aim of the Study
- To investigate the influence of the herbal products Elixir-3, Elixir-9 and Elixir-11 on the replication of herpes viruses.
- To investigate the influence of these extracts on the extracellular virions of virus models.
- To recommend the most active elixir for the next virological investigations with a view to its introduction to the practice.
Analysis of the Problem (with abridgements)
Out of the well-known 8 human herpes viruses, the most widespread of them herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) are causative agnets of infections with wide spectrum of clinical manifestations from slight eruption on the skin and mucosa, to ophthalmic damages and to generalized infections affecting the central nervous system with lethal exit. Relationship was established between these infections and the difficult clinical symptoms in patients suffering from AIDS. An increase in the frequency of recurrences is recorded throughout the world. Minimum number of pharmaceuticals for systemic treatment is applied in the therapy of virus infections because of the obligate intracellular parasitism of the viruses.
...The advantage of natural substances with antivirus activity over the synthetic preparations consists of the unusually difficult creation of virus strains resistant to their activity (…)
In the last three years teams in the Institute of Microbiology at the Bulgarian Academy of Sciences, in the Clinic of children’s surgery, Medical University in Sofia, in the University paediatric hospital and in the Veterinary faculty at the Forestry University in Sofia actively analyse (…) preparations in the series Elixir for the human medicine. High values of activity against a considerable number of pathogenic strains of microorganisms were found in some of these extracts. Clinical experiments were carried out with positive result concerning HIV-positive patients and patients with chronic infections of hepatitis B and C.
Conclusions
The experiments performed up to now to determine the antiherpes potential of the elixirs subjected to analysis revealed the following features:
- Low toxicity toward stable cell lines. Elixirs 3, 9 and 11 did not induce visible changes in the cellular monolayer at dilution 1:100 of the initial products prescribed for patients to be used per os.
- Clearly expressed inhibitory activity of Elixir-3 toward the replication of HSV-1, strain DA and strain BA of HSV-2, applied in non-toxic concentration for the cells. Replication of both virus models was inhibited by 35 and 21 %, respectively.
- Elixir-3 and Elixir-9 almost completely inhibited the extracellular herpes simplex virus type 1 over 98 % for a long time.
- The three tested extracts possess similar strongly expressed virocidal effect with respect to herpes simplex virus type 2.
February 28, 2002 , Sofia,
Head of the research team: Dr. St. Shishkov